An internationally acclaimed film-maker and artist, Madhusudhanan's artistic practice flows seamlessly across media. His fascination with images, especially the advent of the moving image and its place in human history, is reflected in a series of films, paintings, drawings, video art and sculptural installations. The feature film 'Bioscope' (2008) is one of his foremost works. It is based on the journey of the then new art form of cinema during the colonial India. He is deeply concerned with issues of war, colonisation, India's film history and manmade borders. Marxism and Buddhism have been decisive influences on his art.

 The Marx Archive: The Logic of Disappearance' is an ongoing project comprising of drawings, sculptural installations and video. From this series, 90 charcoal drawings were shown.at the Kochi Muziris Biennale, 2014, curated by Jitish Kallat. Selection from the same series and a new series called The Penal Colony' were shown at the Venice Biennale, 2015, curated by Okwui Enwezor. His films have been awarded several international and national awards and have been shown extensively in film festivals, art galleries, museums, including Museum of Modern Art (MoMA), New York.